Feeding stimulant (FS) is defined as the substance which has high ingestion rate by fish (Kasumyan andDøving 2003). In aquaculture, dietary supplementation of FS has been commonly practised to pellet-wean(Person-Le Ruyet 1989; Kubitza and Lovshin 1999; Hirt-Chabbert et al. 2012) and improve the feed intake infish (Dias et al. 1997; Xue and Cui 2001; Papatryphon and Soares 2000). However, the taste preference in fish is species-specific (Kasumyan and Døving 2003); therefore, the effective FS should be identified prior to the
dietary supplementation. Betaine, taurine, nucleotides and nucleosides are amongst the commonly known FSfor fish (Kasumyan and Døving 2003; Chatzifotis et al. 2008; Lech and Reigh 2012). In addition, betaine is also known to be a potential feed enhancer for fish, which itself contains no effect of taste stimulant, but it can enhance the flavour of other chemical substances in a mixture (Kasumyan and Døving 2003). Marble goby (Oxyeleotris marmoratus) is a high commercial value fish species in the aquaculture industry