To obtain insight into the saccharification process, the amounts and types of components that were solubilized during enzymatic digestion were monitored. To this end, samples from the sugar beet pulp suspension were taken after several times of incubation. Pulp suspensions were separated into soluble (hydrolysate) and insoluble (pellet) fractions. The masses of all material added to or removed from the system were recorded in order to calculate the mass balance for the saccharification, after analysis of all fractions that were sampled.