Sticky traps were formed from a tile with approximate size of 15 cm×15 cm.
Bread or cake used as bait in center of circular dry adhesive.
Cockroaches caught on the tape, placed as attraction bait, when came for eating.
These traps were embedded after sunset in the appropriate places such as corners of the patient’s room, under dresser, under refrigerators.
The samples were collected at the next night. After transferring the samples to the laboratory, each of them was anesthetized by chloroform.
Firstly, their genera and species with stereo-microscope were identified using specific detection key.
Then each of the samples washed into sterile glass containing 2 mL sterile saline and the resulting suspension was cultured.
Next, samples washed in ethylic alcohol lasting 2 min for removing the external contamination.